Journal: International Journal of Molecular Sciences

Article Title: Peptide-Guided Photodynamic Therapy via Integrin αvβ6 in Pancreatic Cancer

doi: 10.3390/ijms27041838

Figure Lengend Snippet: ( a ) Expression of αvβ6 and αvβ3 integrins in pancreatic cancer cell lines. ( a ) Normalized fluorescence measurements showing integrin expression levels in BxPc3, MiaPaca2, and Panc1 cells. BxPc3 cells display significantly higher αvβ6 expression compared with MiaPaca2 and Panc1, whereas αvβ3 expression is detectable at lower levels with a different expression profile across cell lines. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by post hoc multiple comparison testing; differences were considered statistically significant at *** p < 0.001 (* p < 0.05 where indicated). ( b ) Western blot results showing β6 integrin expression levels normalized to protein concentration (pg β/µg protein) across different PDAC cell lines. BxPc3 expresses high levels of β6 (1411 pg/µg), MiaPaca2 and Panc1 expresses show no detectable levels of this integrin.

Article Snippet: Capan1, MiaPaca2, PANC1 and BxPc3 cells were purchased from ATCC (Manassas, VA, USA) All cell lines were cultured in RPMI medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 IU/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Expressing, Fluorescence, Comparison, Western Blot, Protein Concentration

Journal: International Journal of Molecular Sciences

Article Title: Peptide-Guided Photodynamic Therapy via Integrin αvβ6 in Pancreatic Cancer

doi: 10.3390/ijms27041838

Figure Lengend Snippet: ( a ) BxPc3 spheroids form stable, viable 3D structures, while MIAPaca2 cells disaggregate by day 7. ( b ) Untreated spheroid before treatment; ( c ) same spheroid after drug treatment with drug in red, nuclei in blue, and membranes in green.

Article Snippet: Capan1, MiaPaca2, PANC1 and BxPc3 cells were purchased from ATCC (Manassas, VA, USA) All cell lines were cultured in RPMI medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 IU/mL penicillin, and 100 μg/mL streptomycin.

Techniques: